Can I read Cryopreservation of Tissue Engineered Constructs for Bone on EtoBox?

Cryopreservation of Tissue Engineered Constructs for Bone by Michelle D. Kofron; Natalie C. Opsitnick; Mohamed A. Attawia; Cato T. Laurencin is a Medicine article available to read on EtoBox.

What is Cryopreservation of Tissue Engineered Constructs for Bone about?

## Abstract The large‐scale clinical use of tissue engineered constructs will require provisions for its mass availability and accessibility. Therefore, it is imperative to understand the effects of low temperature (–196°C) on the tissue engineered biological system. Initial studies used samples of the osteoblast‐like cell line (SaOS‐2) adhered to a two‐dimensional poly(lactide‐__co__‐glycolide) thin film (2D‐PLAGA) or a three‐dimensional poly(lactide‐__co__‐glycolide) sintered microsphere matrix (3D‐PLAGA) designed for bone tissue engineering. Experimental samples were tested for their ability to maintain cell viability, following low temperature banking for one week, in solutions of the penetrating cryoprotective agents, dimethylsulfoxide (DMSO), ethylene glycol, and glycerol. Results indicated the DMSO solution yielded the greatest percent cell survival for SaOS‐2 cells adhered to both the 2D‐ and 3D‐PLAGA scaffolds; therefore, DMSO was used to cryopreserve mineralizing primary rabbit osteoblasts cells adhered to 2D‐PLAGA matrices for 35 days. Results indicated retention of the extracellular matrix architecture as no statistically significant difference in the pre‐ and post‐thaw

Who reads Cryopreservation of Tissue Engineered Constructs for Bone?

Author

Michelle D. Kofron; Natalie C. Opsitnick; Mohamed A. Attawia; Cato T. Laurencin

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0736-0266)

Published

2003

Language

EN

Field

Medicine (Health Sciences)