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Accumulation of porphobilinogen deaminase, uroporphyrinogen decarboxylase, and alpha- and beta-globin mRNAs during differentiation of mouse erythroleukemic cells. Effects of succinylacetone. by B Grandchamp; C Beaumont; H de Verneuil; Y Nordmann is a scholarly article available to read on EtoBox.

What is Accumulation of porphobilinogen deaminase, uroporphyrinogen decarboxylase, and alpha- and beta-globin mRNAs during differentiation of mouse erythroleukemic cells. Effects of succinylacetone. about?

We have monitored, during the dimethyl sulfoxide (MezSO)-induced differentiation of MEL cells, the accumulation of mRNAs encoding two enzymes of the heme biosynthetic pathway, namely porphobilinogen deaminase and uroporphyrinogen decarboxylase. 1. Our results demonstrate that the induction of these two enzymes is accounted for by a coordinate increase in their corresponding mRNAs, as estimated by hybridization with specific cloned cDNA probes. These events occur early during the differentiation process and precede the accumulation of a-and @globin mRNAs. ## 2. Blocking the heme biosynthetic pathway with succinylacetone does not appear to modify the MeaSOmediated increase of porphobilinogen deaminase and uroporphyrinogen decarboxylase mRNAs although succinylacetone has been shown to prevent the induction of immunoreactive porphobilinogen deaminase as well as its enzymatic activity (Beaumont, C., Deybach, J. C., Grandchamp, B., Da Silva, V., de Verneuil, H., and Nordmann, Y. (1984) Exp. Cell Res. 154, 474-484). 3. Heme depletion resulting from the presence of succinylacetone in the culture medium reduces the extent of the Me,SO-mediated accumulation of a-and @globin mRNAs, and this e

Author

B Grandchamp; C Beaumont; H de Verneuil; Y Nordmann

Publisher

Elsevier BV

Published

1985

Language

EN