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Effect of Heat Shock on Intracellular Calcium Mobilization in Neuroblastoma × Glioma Hybrid Cells by Shigenori Katayama; Hisato Shuntoh; Shogo Matsuyama; Chikako Tanaka is a Neuroscience article available to read on EtoBox.

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Abstract: The effect of heat shock on agonist‐stimulated intracellular Ca^2+^ mobilization and the expression of heat shock protein 72 (hsp72) in neuroblastoma × glioma hybrid cells (NG 108–15 cells) were examined. Hsp72 was expressed at 6 h after heat shock (42.5°C, 2 h), reached a maximum at 12 h, and decreased thereafter. Bradykinin‐induced [Ca^2+^], rise was attenuated to 28% of control by heat shock at 2 h after heat shock, and reversion to the control level was seen 12 h later. When the cells were treated with quercetin or antisense oligodeoxyribonucleotide against hsp72 cDNA, the synthesis of hsp72 was not induced by heat shock, whereas bradykinin‐induced [Ca^2+^]~i~ rise was abolished and the [Ca^2+^]~i~ rise was not restored. Recovery from this stressed condition was evident when cells were stimulated by the Ca^2+^‐ATPase inhibitor thapsigargin, even in the presence of either quercetin or antisense oligodeoxyribonucleotide. Inositol 1,4,5‐trisphosphate (IP~3~) production was not altered by heat shock at 12 h after heat shock, whereas IP~3~ receptor binding activity was reduced to 45.3%. In the presence of quercetin or antisense oligodeoxyribonucleotide, IP~3~ receptor bin

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Author

Shigenori Katayama; Hisato Shuntoh; Shogo Matsuyama; Chikako Tanaka

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1994

Language

EN

Field

Neuroscience (Life Sciences)