Can I read Systematic Screening of Protein Modifications in Four Kinases Using Affinity Enrichment and Mass Spectrometry Analysis with Unrestrictive Sequence Alignment on EtoBox?

Systematic Screening of Protein Modifications in Four Kinases Using Affinity Enrichment and Mass Spectrometry Analysis with Unrestrictive Sequence Alignment by Kai Zhang; Yixin Zhu; Xiwen He; Yukui Zhang is a Chemistry article available to read on EtoBox.

What is Systematic Screening of Protein Modifications in Four Kinases Using Affinity Enrichment and Mass Spectrometry Analysis with Unrestrictive Sequence Alignment about?

Protein kinases transfer phosphate groups from ATP to substrate proteins, they are known to be involved in diverse cellular processes. They are also important therapeutic targets in pharmaceutical design. Previous studies indicated that multiple post-translational modifications (PTMs) exist in kinases in addition to phosphorylation, and these PTMs play an important role in regulating kinases activities. Nevertheless, a comprehensive analysis for PTMs of kinases is insufficient due to technical limitations, which prevent us from better understanding their functional regulation. Here, we have developed a novel strategy that combines glutathione S-transferase tag affinity enrichment with nano-liquid chromatography coupled with tandem mass spectrometry analysis and non-restrictive protein sequence alignment for identification of diverse PTMs in four yeast kinases. The method allows us to enrich and analyze the entire protein isomers and to minimize the loss of all isomers of protein sample during protein purification. In our study, nineteen phosphorylation sites and several other types of PTMs sites were localized in 4 protein kinases. In addition, we found that some interesting mass s

Who reads Systematic Screening of Protein Modifications in Four Kinases Using Affinity Enrichment and Mass Spectrometry Analysis with Unrestrictive Sequence Alignment?

Author

Kai Zhang; Yixin Zhu; Xiwen He; Yukui Zhang

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)

Published

2011

Language

EN

Field

Chemistry (Physical Sciences)