About this Medicine article

Distinct cellular origins of primary effusion lymphoma with and without EBV infection by Rifat Hamoudi; Tim C Diss; Eric Oksenhendler; Langxing Pan; Antonino Carbone; Valeria Ascoli; Chris Boshoff; Peter Isaacson; Ming-Qing Du is a Medicine article available to read on EtoBox.

Kaposi's sarcoma-associated herpesvirus (KSHV) is associated with three distinct lymphoproliferative disorders: primary effusion lymphoma (PEL), multicentric Castleman's disease (MCD) and germinotropic lymphoproliferative disorder (GLD). KSHV positive lymphocytes in GLD and in most cases of PEL are co-infected by Epstein-Barr virus (EBV) and these viral double positive cells harbour mutated rearranged immunoglobulin (Ig) genes, suggesting that they originate from germinal centre or post-germinal centre B-cells. In contrast, KSHV positive cells in MCD are invariably negative for EBV, do not carry Ig gene mutation and are believed to originate from naïve IgMlambda expressing B-cells. Interestingly, one EBV negative PEL (BC3) also lacks Ig gene mutation, raising the question whether KSHV preferentially targets naïve B-cells in the absence of EBV. We compared the cellular origin of PEL with and without EBV infection by analysis of Ig gene mutation. High molecular weight DNA from 17 PELs was subjected to PCR of the rearranged Ig heavy and light chain genes. Successful amplification was achieved from eight cases (four EBV positive and four EBV negative) and the PCR products were sequence

Author

Rifat Hamoudi; Tim C Diss; Eric Oksenhendler; Langxing Pan; Antonino Carbone; Valeria Ascoli; Chris Boshoff; Peter Isaacson; Ming-Qing Du

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0145-2126)

Published

2004

Language

EN

Field

Medicine (Health Sciences)