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A nonconsensus octamer‐recognition sequence (TAATGARAT‐motif) identifies a novel DNA binding protein in human merkel cell carcinoma cell lines by J. A. F. Thomson; J. H. Leonard; K. McGregor; R. A. Sturm; P. G. Parsons is a Medicine article available to read on EtoBox.

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## Abstract We have established a number of cell lines from Merkel cell carcinoma (MCC) of the skin. In many respects these cell lines resemble those established from small cell lung cancer (SCLC) and it is difficult to differentiate between metastatic MCC and SCLC on morphological or histochemical criteria. Both are thought to be neuroendocrine tumours and may express a number of neuroendocrine markers. SCLC cell lines express the octamer‐DNA binding transcription factor __brn__‐2 gene products N‐Oct 3 and N‐Oct 5, which are restricted to the neuroectodermal cell lineage. In DNA binding studies using a consensus octamer recognition site we have found that 4 of 8 MCC cell lines examined expressed __brn__‐2 in at least trace amounts compared with 3 SCLC cell lines which all expressed __brn__‐2 proteins at high levels. Moreover, these DNA binding studies were extended by using a high‐affinity __brn__‐2 recognition site related to the degenerate octamer TAATGARAT‐motif. This identified a novel DNA binding protein in a subset of MCC cell lines. The protein was absent from the three SCLC cell lines, melanoma cells and brain tissue. This binding activity, which we term Merkel cell DNA nu

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Author

J. A. F. Thomson; J. H. Leonard; K. McGregor; R. A. Sturm; P. G. Parsons

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1994

Language

EN

Field

Medicine (Health Sciences)