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Method Development and Validation for Isoflavones in Soy Germ Pharmaceutical Capsules Using Micellar Electrokinetic Chromatography by Gustavo Amadeu Micke; Neide Mitsue Fujiya; Fernando Gustavo Tonin; Ana Carolina de Oliveira Costa; Marina Franco Maggi Tavares is a Chemistry article available to read on EtoBox.

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The separation of six soy isoflavones (Glycitein, Daidzein, Genistein, Daidzin, Glycitin and Genistin) was approached by a 3(2) factorial design studying MEKC electrolyte components at the following levels: methanol (MeOH; 0-10%) and sodium dodecylsulfate (SDS; 20-70 mmol L(-1)); sodium tetraborate buffer (STB) concentration was kept constant at 10 mmol L(-1). Nine experiments were performed and the apparent mobility of each isoflavone was computed as a function of the electrolyte composition. A novel response function (RF) was formulated based on the production of the mobility differences, mobility of the first and last eluting peaks and the electrolyte conductance. The inspection of the response surface indicated an optimum electrolyte composition as 10 mmol L(-1) STB (pH 9.3) containing 40 mmol L(-1) SDS and 1% MeOH promoting baseline separation of all isoflavones in less than 7.5 min. The proposed method was applied to the determination of total isoflavones in soy germ capsules from four different pharmaceutical laboratories. A 2h extraction procedure with 80% (v/v) MeOH under vortexing at room temperature was employed. Peak assignment of unknown isoflavones in certain samples

Who reads Method Development and Validation for Isoflavones in Soy Germ Pharmaceutical Capsules Using Micellar Electrokinetic Chromatography?

Author

Gustavo Amadeu Micke; Neide Mitsue Fujiya; Fernando Gustavo Tonin; Ana Carolina de Oliveira Costa; Marina Franco Maggi Tavares

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)

Published

2006

Language

EN

Field

Chemistry (Physical Sciences)