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Can I read Cleanup Procedure for Determination of Aflatoxins in Major Agricultural Commodities by Liquid Chromatography on EtoBox?
Cleanup Procedure for Determination of Aflatoxins in Major Agricultural Commodities by Liquid Chromatography by Sobolev, Victor S; Dorner, Joe W is a Chemistry article available to read on EtoBox.
What is Cleanup Procedure for Determination of Aflatoxins in Major Agricultural Commodities by Liquid Chromatography about?
## Abstract A simple, fast, reliable, and inexpensive chemical cleanup procedure was developed for quantitation of aflatoxins in major important agricultural commodities by liquid chromatography (LC). Aflatoxins were extracted from a ground sample with methanol–water (80 + 20, v/v), and after a single cleanup step on a minicolumn packed with basic aluminum oxide, they were quantitated by LC equipped with a C18 column, photochemical reactor, and fluorescence detector. Water–methanol–1-butanol (1400 + 720 + 25, v/v/v) served as the mobile phase. Recoveries of aflatoxins B1, B2, G1, and G2 from peanuts spiked at 5.0, 2.5, 7.5, and 2.5 μg/kg were 87.2 ± 2.3, 82.0 ± 0.8, 80.0 ± 1.8, and 80.4 ± 2.8%, respectively. Similar recoveries, precision, and accuracy were achieved for corn, cottonseed, almonds, Brazil nuts, pistachios, and walnuts. The quantitation limit for aflatoxin B1 was 1 μg/kg. The minimal cost of the minicolumn allows for substantial savings compared with available commercial aflatoxin cleanup devices.
Who reads Cleanup Procedure for Determination of Aflatoxins in Major Agricultural Commodities by Liquid Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Sobolev, Victor S; Dorner, Joe W
- Publisher
- Association of Official Analytical Chemists; The Journal of AOAC International; AOAC International; Oxford University Press (OUP) (ISSN 1060-3271)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)