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Matrix Metalloproteinases, Their Tissue Inhibitors and Colorectal Cancer Staging by Dr E. A. Baker; F. G. Bergin; D. J. Leaper is a Medicine article available to read on EtoBox.

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## Abstract ## Background Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are important in tumour invasion and metastasis. The levels of MMPs, TIMPs and total MMP activity were compared in paired colorectal tumour (n = 50) and normal tissue (n = 49) samples and correlated with clinical and pathological staging. ## Methods Gelatin zymography (MMP-2 and MMP-9), enzyme-linked immunosorbent assays (MMP-1, MMP-3, TIMP-1 and TIMP-2) and quenched fluorescent substrate hydrolysis (total MMP activity) were employed in resection specimens from 50 patients, four with adenomas and 46 with colorectal cancer. ## Results The levels of active MMP-2 and MMP-9 and total MMP-1, MMP-3 and TIMP-1 were significantly greater in tumour tissue than in normal colon (e.g. TIMP-1 tumour median 72 (range 25–351) versus normal 26 (4–107) ng per mg total protein content; P < 0·05); however, TIMP-2 levels were significantly greater in normal tissue (P < 0·05). Total MMP activity was significantly greater in tumour than in normal tissue (15 025 (1750–174 400) versus 7250 (750–354 650) pmol l−1 min−1 mg protein−1; P < 0·05). Correlations were found between both MMP and TIMP levels and pathologi

Who reads Matrix Metalloproteinases, Their Tissue Inhibitors and Colorectal Cancer Staging?

Author

Dr E. A. Baker; F. G. Bergin; D. J. Leaper

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Oxford University Press (OUP) (ISSN 0007-1323)

Published

2000

Language

EN

Field

Medicine (Health Sciences)