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Development of an efficient insecticide substrate and inhibitor screening system of insect P450s using fission yeast by Xiang Li; Lianyun Lin; Zhi Li; Hadiatullah Hadiatullah; Shishir Sharma; He Du; Xin Yang; Wei Chen; Shijun You; Matthias Bureik; Zhiguang Yuchi is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Development of an efficient insecticide substrate and inhibitor screening system of insect P450s using fission yeast about?
Metabolic resistance is one of the most frequent mechanisms of insecticide resistance, characterized by an increased expression of several important enzymes and transporters, especially cytochrome P450s (CYPs). Due to the large number of P450s in pests, determining the precise relationship between these enzymes and the insecticide substrates is a challenge. Herein, we developed a luminescence-based screening system for efficient identification of insecticide substrates and insect P450 inhibitors. We recombinantly expressed Bemisia tabaci CYP6CM1vQ (Bt CYP6CM1vQ) in the fission yeast Schizosaccharomyces pombe and subsequently permeabilized the yeast cells to convert them into “enzyme bags”. We exploited these enzyme bags to screen the activity of twelve luciferin substrates and identified Luciferin-FEE as the optimal competing probe that was further used to characterize the metabolism of eight candidate commercial insecticides. Among them, Bt CYP6CM1vQ exhibited notable activity against pymetrozine and imidacloprid. Their binding modes were predicted by homology modeling and molecular docking, revealing the mechanisms of the metabolism. We also tested the inhibitory effect of eight
Who reads Development of an efficient insecticide substrate and inhibitor screening system of insect P450s using fission yeast?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Xiang Li; Lianyun Lin; Zhi Li; Hadiatullah Hadiatullah; Shishir Sharma; He Du; Xin Yang; Wei Chen; Shijun You; Matthias Bureik; Zhiguang Yuchi
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)