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Horizontal gene transfer (HGT): A driving force for resistance dissemination in gut microbiota by Ibrahim Farouk Farag; Magda Mahmoud Hagag is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Horizontal gene transfer (HGT): A driving force for resistance dissemination in gut microbiota about?

Viperidae venom is a rich source of bioactive molecules. Proteomic analysis was used to identify molecules from whole venom. Three biomolecules newly isolated from Cerastes cerastes venom have been analyzed by Nano-liquid Chromatography and Mass Spectrometry. After reduction, alkylation and double hydrolysis of the molecules by Lysine-C endopeptidase and trypsin, LC-MALDI-MS/MS was performed. The proteins were identified by mining the database using the NCBInr protein. Obtained results revealed the presence of another phospholipase activity (CC2-PLA2) which shares 51% of homology sequence with the previous purified PLA2 from the same venom. CC2-PLA2 exhibits antiplatelet activity leading to a coagulation blockade. CCSV-MPase, a non-haemorrhagic Zn 2+ -metalloproteinase, reduced significantly the plasmatic fibrinogen level and hydrolyzed only its B( chain. However, CC3-SPase (a serine protease) could be an ( ( fibrinogenase triggering human platelet aggregation. In the presence of human plasma deficient in Factor II, CC3-SPase revealed a blood-clotting activity comparable to that obtained with normal plasma. However, the clot so obtained was soluble indicating that CC3-SPase does no

Who reads Horizontal gene transfer (HGT): A driving force for resistance dissemination in gut microbiota?

Author

Ibrahim Farouk Farag; Magda Mahmoud Hagag

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0958-1669)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)