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Amine Oxidases in Clinical Research discusses the functions of amine oxidases in the rapid development of medical and biological research. Composed of 13 chapters, the book focuses on amine oxidases, including their key role in the metabolism of biogenic amines and other nitrogenous compounds in diseases and human health. The book considers primarily the enzymes denoted as EC 1.4.3.4 (flavin amine oxidases or monoamine oxidases) and EC 1.4.3.6 (copper-containing amine oxidase or diamine oxidases). To measure the estimates of amine oxidase activity, several methods and a number of requirements are presented. A description of the distribution and intracellular localization of amine oxidase follows. The methods of isolation and purification of amine oxides from different sources and the results of these various methods are explained. The text also considers the properties of the purified amine oxidases: the different methods of isolation and purification; storage conditions; and analytical techniques. Care is given when examining amine oxidases, because these may exhibit considerable differences in substrate specificity even if their physicochemical properties are similar. Inhibition

Author

V. Z. Gorkin

Publisher

Elsevier Science & Technology Books

Published

1983

Language

EN

ISBN

9780080255231