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Molecular characterization of CXCR–4: A potential brain tumor-associated gene by Sehgal, Anil; Ricks, Sherianne; Boynton, Alton L.; Warrick, Jami; Murphy, Gerald P. is a Medicine article available to read on EtoBox.

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## Background and objectives: We have previously reported the isolation of a g protein-coupled receptor, cxcr-4, that is overexpressed in glioblastoma multiforme tumor tissue (gmtt), as compared to normal brain tissue (nbt). ## Methods: Gene-specific rt-pcr, northern blotting, and in situ hybridization techniques were used to study its expression in a variety of normal tissues, tumor tissues, and cell lines, as well as during development. antisense cxcr-4 was overexpressed in glioblastoma cells to study its effect on cell proliferation. ## Results: Gene-specific rt-pcr analysis indicated that the cxcr-4 gene is overexpressed in several malignant glioma tissues, breast tumor tissues and cell lines. northern blot analysis indicated that cxcr-4 is expressed at high levels in certain leukemias, uterine cancer, and burkitt's lymphoma cell line. the occipital and temporal lobe showed high levels of cxcr4 in normal human brain. the cxcr-4 gene was expressed in all organs in the early stages of development (days 8-10). in adult mouse, cxcr-4 is expressed only in brain, spinal cord, bone marrow, and pituitary gland. antisense cxcr-4 overexpression in glioblastoma cells caused inhibition of

Who reads Molecular characterization of CXCR–4: A potential brain tumor-associated gene?

Author

Sehgal, Anil; Ricks, Sherianne; Boynton, Alton L.; Warrick, Jami; Murphy, Gerald P.

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-4790)

Published

1999

Language

EN

Field

Medicine (Health Sciences)