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1) The study optimized in vitro liquid culture of the entomopathogenic nematode Steinernema jeffreyense using shake flasks. 2) They found that using 30 ml of liquid medium in flasks on an orbital shaker resulted in 86% recovery and a yield of over 121,000 infective juveniles per ml after 14 days. 3) Starting with a lower initial inoculum density of 1,000 infective juveniles per ml led to higher recovery and yield than higher densities. 4) Infective juveniles produced in vitro showed higher pathogenicity a

Author

Wisnu Ardani

Language

EN