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Effect of Borate Buffer on the Photolysis of Riboflavin in Aqueous Solution by Iqbal Ahmad; Sofia Ahmed; Muhammad Ali Sheraz; Faiyaz H.M. Vaid is a Medicine article available to read on EtoBox.

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The photolysis of riboflavin (RF) in the presence of borate buffer (0.1-0.5 M) at pH 8.0-10.5 has been studied using a specific multicomponent spectrophotometric method for the determination of RF and photoproducts, formylmethylflavin (FMF), lumichrome (LC) and lumiflavin (LF). The overall first-order rate constants for the photolysis of RF (1.55-4.36 Â 10 À2 min À1 ) and the rate constants for the formation of FMF (1.16-3.52 Â 10 À2 min À1 ) and LC (0.24-0.84 Â 10 À2 min À1 ) have been determined. The values of all these rate constants decrease with an increase in buffer concentration suggesting the inhibition of photolysis reaction by borate species. The kinetic data support the formation of a RF-borate complex involving the ribityl side chain to cause the inhibition of photolysis. The second-order rate constants for the borate inhibited reaction range from 1.17-3.94 Â 10 À2 M À1 min À1 . The log k-pH profiles for the reaction at various buffer concentrations indicate a gradual increase in rate, with pH, up to 10 followed by a decrease in rate at pH 10.5 probably due to ionization of RF and quenching of fluorescence by borate species. A graph of second-order rate constants agains

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Author

Iqbal Ahmad; Sofia Ahmed; Muhammad Ali Sheraz; Faiyaz H.M. Vaid

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1011-1344)

Published

2008

Language

EN

Field

Medicine (Physical Sciences)