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Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103 by Yan-Bin Teng; Yong-Liang Jiang; Yong-Xing He; Wei-Wei He; Fu-Ming Lian; Yuxing Chen; Cong-Zhao Zhou is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103 about?
## Abstract ## Background The carbonic anhydrases (CAs) are involved in inorganic carbon utilization. They have been classified into six evolutionary and structural families: α-, β-, γ-, δ-, ε-, ζ- CAs, with β-CAs present in higher plants, algae and prokaryotes. The yeast __Saccharomyces cerevisiae__ encodes a single copy of β-CA Nce103/YNL036W. ## Results We determined the crystal structure of Nce103 in complex with a substrate analog at 2.04 Å resolution. It assembles as a homodimer, with the active site located at the interface between two monomers. At the bottom of the substrate pocket, a zinc ion is coordinated by the three highly conserved residues Cys57, His112 and Cys115 in addition to a water molecule. Residues Asp59, Arg61, Gly111, Leu102, Val80, Phe75 and Phe97 form a tunnel to the bottom of the active site which is occupied by a molecule of the substrate analog acetate. Activity assays of full length and two truncated versions of Nce103 indicated that the N-terminal arm is indispensable. ## Conclusion The quaternary structure of Nce103 resembles the typical plant type β-CAs of known structure, with an N-terminal arm indispensable for the enzymatic activity. Comparative
Who reads Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103?
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- Author
- Yan-Bin Teng; Yong-Liang Jiang; Yong-Xing He; Wei-Wei He; Fu-Ming Lian; Yuxing Chen; Cong-Zhao Zhou
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6807)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)