Can I read Highly Efficient, Selective and Sensitive Molecular Screening of Acetylcholinesterase Inhibitors of Natural Origin by Solid-phase Extraction-liquid Chromatography/electrospray Ionisation-octopole-orthogonal Acceleration Time-of-flight-mass Spectrometry and Novel Thin-layer Chromatography-based Bioautography on EtoBox?

Highly Efficient, Selective and Sensitive Molecular Screening of Acetylcholinesterase Inhibitors of Natural Origin by Solid-phase Extraction-liquid Chromatography/electrospray Ionisation-octopole-orthogonal Acceleration Time-of-flight-mass Spectrometry and Novel Thin-layer Chromatography-based Bioautography by Tomasz Mroczek is a Chemistry article available to read on EtoBox.

What is Highly Efficient, Selective and Sensitive Molecular Screening of Acetylcholinesterase Inhibitors of Natural Origin by Solid-phase Extraction-liquid Chromatography/electrospray Ionisation-octopole-orthogonal Acceleration Time-of-flight-mass Spectrometry and Novel Thin-layer Chromatography-based Bioautography about?

Highly efficient, selective and sensitive molecular screening of natural acetylcholinesterase (AChE) inhibitors was developed and comprised optimized pressurized liquid extraction (PLE) of plant materials followed by highly selective solid-phase extraction (SPE) using Oasis HLB cartridges. Pure alkaloidal fractions were analyzed by a newly developed high-performance liquid-chromatography (HPLC) on a 3 m Atlantis HILIC silica stationary phase combined with recently introduced electrospray ionisation (ESI) octopole-orthogonal acceleration time-of-flight (oa TOF)-mass spectrometry (MS) with high mass accuracy (about 2 ppm) and high sensitivity (absolute limit of detection (LOD) for galanthamine was about 43 fg at signal-to-noise 13:1). Moreover, a newly developed and validated TLC-bioautography permit galanthamine sensitivities at pg levels. In this way, more potent than galanthamine AChE inhibitor namely 1,2-dihydrogalanthamine in Narcissus jonquilla 'Pipit' extract could be found (with IC 50 value 0.19 M lower of about 42% than that of galanthamine).

Who reads Highly Efficient, Selective and Sensitive Molecular Screening of Acetylcholinesterase Inhibitors of Natural Origin by Solid-phase Extraction-liquid Chromatography/electrospray Ionisation-octopole-orthogonal Acceleration Time-of-flight-mass Spectrometry and Novel Thin-layer Chromatography-based Bioautography?

Author

Tomasz Mroczek

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)