Can I read Development of aptamer-based inhibitors for CRISPR/Cas system on EtoBox?

Development of aptamer-based inhibitors for CRISPR/Cas system by Jing Zhao; Rika Inomata; Yoshio Kato; Makoto Miyagishi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Development of aptamer-based inhibitors for CRISPR/Cas system about?

## Abstract The occurrence of accidental mutations or deletions caused by genome editing with CRISPR/Cas9 system remains a critical unsolved problem of the technology. Blocking excess or prolonged Cas9 activity in cells is considered as one means of solving this problem. Here, we report the development of an inhibitory DNA aptamer against Cas9 by means of in vitro selection (systematic evolution of ligands by exponential enrichment) and subsequent screening with an __in vitro__ cleavage assay. The inhibitory aptamer could bind to Cas9 at low nanomolar affinity and partially form a duplex with CRISPR RNA, contributing to its inhibitory activity. We also demonstrated that improving the inhibitory aptamer with locked nucleic acids efficiently suppressed Cas9-directed genome editing in cells and reduced off-target genome editing. The findings presented here might enable the development of safer and controllable genome editing for biomedical research and gene therapy.

Who reads Development of aptamer-based inhibitors for CRISPR/Cas system?

Author

Jing Zhao; Rika Inomata; Yoshio Kato; Makoto Miyagishi

Publisher

Oxford University Press; London: Information Retrieval; Oxford: Oxford University Press; Oxford University Press (OUP) (ISSN 0305-1048)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)