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Experimentally induced monensin-resistant Eimeria tenella and membrane fluidity of sporozoites by Zhuo Wang; Jianzhong Shen; Xun Suo; Shiyun Zhao; Xingyuan Cao is a Immunology and Microbiology article available to read on EtoBox.

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Two resistant lines of Eimeria tenella (H) to monensin were developed after 35 passages in chickens medicated with 100-125 ppm or 125-200 ppm monensin in the diet. Drug sensitivity of the induced lines to different level drugs were estimated with mean lesion scores (LS), mean oocyst productions (OP), percentage optimum anticoccidial activity (POAA), reduction of lesion scores (RLS), relative oocyst production (ROP), anticoccidial index (ACI) and global index (GI), respectively. Membrane fluidity of sporozoites of the sensitive line (i.e. the parent line, coded as MON-S (S) ) and two resistant lines (coded as MON-R (S) -1 and MON-R (S) -2) with and without in vitro exposure to monensin were determined. Membrane fluidity of MON-R (S) -1 and MON-R (S) -2 were significantly lower than that of MON-S (S) . In vitro exposure to monensin significantly increased membrane fluidity of MON-S (S) , but had a much less effect on those of MON-R (S) -1 and MON-R (S) -2. Sporozoits of the MON-S (S) and MON-R (S) -2 with or without in vitro exposure to monensin were examined by SEM, and the sensitive sporozoites (MON-S (S) ) appeared swollen and bulgy after treatment with monensin, while there was n

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Author

Zhuo Wang; Jianzhong Shen; Xun Suo; Shiyun Zhao; Xingyuan Cao

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)

Published

2006

Language

EN

Field

Immunology and Microbiology (Life Sciences)