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Impact of Delayed Processing of Positive Blood Cultures on Organism Detection: a Prospective Multi-centre Study by Tamalee Roberts; Arjun Chandna; Wanitda Watthanaworawit; Areerat Thaiprakong; Sona Soeng; Manivone Simmalavong; Phonelavanh Phoumin; Weerawut Saengchun; Nongyao Khatta; Pattaraporn Hinfonthong; Napaporn Kaewpundoem; Sue J. Lee; Carlo Perrone; Ben Amos; Paul Turner; Elizabeth A. Ashley; Clare L. Ling is a Medicine article available to read on EtoBox.
What is Impact of Delayed Processing of Positive Blood Cultures on Organism Detection: a Prospective Multi-centre Study about?
## Background Blood cultures remain the gold standard investigation for the diagnosis of bloodstream infections. In many locations, quality-assured processing of positive blood cultures is not possible. One solution is to incubate blood cultures locally, and then transport bottles that flag positive to a central reference laboratory for organism identification and antimicrobial susceptibility testing. However, the impact of delay between the bottle flagging positive and subsequent sub-culture on the viability of the isolate has received little attention. ## Methods This study evaluated the impact of delays to sub-culture (22 h to seven days) in three different temperature conditions (2–8 °C, 22–27 °C and 35 ± 2 °C) for bottles that had flagged positive in automated detection systems using a mixture of spiked and routine clinical specimens. Ninety spiked samples for five common bacterial causes of sepsis (__Escherichia coli__, __Haemophilus influenzae, Staphylococcus aureus, Streptococcus agalactiae__ and __Streptococcus pneumoniae__) and 125 consecutive positive clinical blood cultures were evaluated at four laboratories located in Cambodia, Lao PDR and Thailand. In addition, the u
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- Author
- Tamalee Roberts; Arjun Chandna; Wanitda Watthanaworawit; Areerat Thaiprakong; Sona Soeng; Manivone Simmalavong; Phonelavanh Phoumin; Weerawut Saengchun; Nongyao Khatta; Pattaraporn Hinfonthong; Napaporn Kaewpundoem; Sue J. Lee; Carlo Perrone; Ben Amos; Paul Turner; Elizabeth A. Ashley; Clare L. Ling
- Publisher
- Springer Science and Business Media LLC
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)