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The action of phospholipase C and lipase on black film bilayer membranes by H.Stewart Hendrickson; D.G. Rustad; E.M. Scattergood; D.E. Engle is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is The action of phospholipase C and lipase on black film bilayer membranes about?

Phospholipase C(Q perfringens) added to one side ofa phosphatidyl eholine-phosphatidyl serine-cholesterol (0.7 : 0.3 : 1.0, w/w/w) bilayer membrane resulted in a rapid decrease in membrane resistance which leveled off after several minutes at about one-half its initial value. Pancreatic lipase added at this time resulted in a rapid increase in membrane resistance to a value somewhat higher than the initial. This effect was independent of the side to which the lipase was added, indicating that diglyceride is rapidly equilibrated across both sides of the membrane. When both phospholipase C and lipase were added at zero time to the same or opposite sides, the resistance decreased slightly and then increased to a value higher than the initial. Replacement of phosphatidyl choline by 2-hexadecoxy-3-octadecoxypropyl-phosphonylcholine, a phosphonate analog, resulted in an inhibition of the phospholipase C reaction which was equal to the percent analog. The analog, thus, seemed to act only as inert lipid and did not inhibit hydrolys of the active lipid.

Who reads The action of phospholipase C and lipase on black film bilayer membranes?

Author

H.Stewart Hendrickson; D.G. Rustad; E.M. Scattergood; D.E. Engle

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-3084)

Published

1974

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)