About this document
Mapping Ampicillin Resistance on pBR322 by Tim Hepburn is a document available to read on EtoBox.
The document summarizes an experiment to map the location of the ampicillin resistance gene on the pBR322 plasmid using the Tn5 transposon. Key steps included: 1) Using a suicide phage containing Tn5 to insert the transposon into E. coli cells containing pBR322, 2) Selecting for kanamycin resistance to identify transposon insertions, 3) Isolating plasmid DNA and identifying ampicillin sensitive mutants, 4) Digesting the plasmid DNA and running it on a gel to determine fragment sizes and map the transposon i
- Author
- Tim Hepburn
- Language
- EN