Can I read Autoinduced synthesis of colicin E2 on EtoBox?

Autoinduced synthesis of colicin E2 by Anthony P. Pugsley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Autoinduced synthesis of colicin E2 about?

Escherichia coli K-12 cells carrying the high copy number plasmid ColE2-P9 and a sfiA-lacZ gene fusion exhibit abnormally high levels of SOS-regulated phi sfiA-lacZ expression. Increased sfiA-lacZ expression is caused by the action of colicin E2, which is a DNase, rather than by the presence of multiple copies of a binding site for LexA protein, the repressor for the sfiA and colicin E2 genes. Expression of sfiA-lacZ was reduced to normal levels if the ColE2+ strain lacked the outer membrane colicin E2 receptor protein (BtuB) or if they carried an increased number of colicin E2 immunity genes. The results suggest that cultures of ColE2+ strains contain a small number of cells which produce colicin which can then enter other, non-producing cells in the culture and cause sufficient damage to the DNA to induce the SOS system. The levels of colicin E2 immunity in the producing cells is presumably sufficient to prevent extensive lethal effects of the colicin, but insufficient to prevent limited endonuclease activity. An important consequence of this phenomenon is that the DNase action of colicin E2 can stimulate its own production.

Who reads Autoinduced synthesis of colicin E2?

Author

Anthony P. Pugsley

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1982

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)