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Volkensin from Adenia volkensii Harms (kilyambiti plant), a type 2 ribosome-inactivating protein : Gene cloning, expression and characterization of its A-chain by Angela Chambery; Antimo Di Maro; Maurilia M. Monti; Fiorenzo Stirpe; Augusto Parente is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Volkensin from Adenia volkensii Harms (kilyambiti plant), a type 2 ribosome-inactivating protein : Gene cloning, expression and characterization of its A-chain about?
Volkensin, a type 2 ribosome‐inactivating protein from the roots of __Adenia volkensii__ Harms (kilyambiti plant) was characterized both at the protein and nucleotide level by direct amino acid sequencing and cloning of the gene encoding the protein. Gene sequence analysis revealed that volkensin is encoded by a 1569‐bp ORF (523 amino acid residues) without introns, with an internal linker sequence of 45 bp. Differences in residues present at several sequence positions (reproduced after repeated protein sequence analyses), with respect to the gene sequence, suggest several isoforms for the volkensin A‐chain. Based on the crystallographic coordinates of ricin, which shares a high sequence identity with volkensin, a molecular model of volkensin was obtained. The 3D model suggests that the amino acid residues of the active site of the ricin A‐chain are conserved at identical spatial positions, including Ser203, a novel amino acid residue found to be conserved in all known ribosome‐inactivating proteins. The sugar binding site 1 of the ricin B‐chain is also conserved in the volkensin B‐chain, whilst in binding site 2, His246 replaces Tyr248. Native volkensin contains two free cysteinyl
Who reads Volkensin from Adenia volkensii Harms (kilyambiti plant), a type 2 ribosome-inactivating protein : Gene cloning, expression and characterization of its A-chain?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Angela Chambery; Antimo Di Maro; Maurilia M. Monti; Fiorenzo Stirpe; Augusto Parente
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)