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Can I read Single-molecule Fingerprinting of Protein-drug Interaction Using a Funneled Biological Nanopore on EtoBox?
Single-molecule Fingerprinting of Protein-drug Interaction Using a Funneled Biological Nanopore by Ki-Baek Jeong; Minju Ryu; Jin-Sik Kim; Minsoo Kim; Jejoong Yoo; Minji Chung; Sohee Oh; Gyunghee Jo; Seong-Gyu Lee; Ho Min Kim; Mi-Kyung Lee; Seung-Wook Chi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Single-molecule Fingerprinting of Protein-drug Interaction Using a Funneled Biological Nanopore about?
In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. Here, we develop a biological nanopore sensor for single-molecule detection of proteins and PDIs using the pore-forming toxin YaxAB. Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. The long funnel-shaped structure and nanofluidic characteristics of the YaxAB nanopore enable the electro-osmotic trapping of diverse folded proteins and high-resolution monitoring of PDIs. Distinctive nanopore event distributions observed in the two-dimensional (ΔI/I~o~-versus-I~N~) plot illustrate the ability of the YaxAB nanopore to discriminate individual small-molecule drugs bound to Bcl-xL from non-binders. Taken together, our results present the YaxAB nanopore as a robust platform for label-free, ultrasensitive, single-molecule detection of PDIs, opening up a possibility for low-cost, highly efficient drug discovery against diverse drug targets.
Who reads Single-molecule Fingerprinting of Protein-drug Interaction Using a Funneled Biological Nanopore?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ki-Baek Jeong; Minju Ryu; Jin-Sik Kim; Minsoo Kim; Jejoong Yoo; Minji Chung; Sohee Oh; Gyunghee Jo; Seong-Gyu Lee; Ho Min Kim; Mi-Kyung Lee; Seung-Wook Chi
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)