About this document
Online HPLC for Process Analytical Tech by laasyagudi is a document available to read on EtoBox.
This document proposes using an offline HPLC system for online analysis of product quality attributes during biopharmaceutical process chromatography. A 2-way/6-port valve is used to facilitate simultaneous automated sampling of the product stream and fraction collection, without sample dilution. This simple and cost-effective configuration was tested on two common separations: aggregates and charge variants of monoclonal antibodies. Process modeling was used to interpolate HPLC data and facilitate pooling
- Author
- laasyagudi
- Language
- EN