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Induction of IL-8 and monoclyte chemoattractant protein-1 by doxorubicin in human small cell lung carcinoma cells by Misako Shibakura; Kenji Niiya; Toru Kiguchi; Isao Kitajima; Masami Niiya; Noboru Asaumi; Nam-ho Huh; Yasunari Nakata; Mine Harada; Mitsune Tanimoto is a Medicine article available to read on EtoBox.

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## Abstract We previously demonstrated doxorubicin‐induced __urokinase__ expression in human H69 SCLC cells by the microarray technique using Human Cancer CHIP version 2 (Takara Shuzo, Kyoto, Japan), in which 425 human cancer‐related genes were spotted on glass plates (Kiguchi et al., Int J Cancer 2001;93:792–7). Microarray analysis also revealed significant induction of __IL‐8__, a member of the CXC chemokines. We have, therefore, extended the observation by testing the effects of doxorubicin on expression of the chemokine family and provide here definitive evidence that doxorubicin induces IL‐8 and MCP‐1, one of the CC chemokines, at least in 2 human SCLC cells, H69 and SBC‐1. IL‐8 antigen levels, measured by ELISA, were markedly increased in both H69 and SBC‐1 conditioned media after doxorubicin treatment, in parallel with mRNA levels; and this was dependent on the dose of doxorubicin. The ribonuclease protection assay, using a multiprobe template set for human chemokines, revealed induction of not only __IL‐8__ but also __MCP‐1__ in doxorubicin‐treated H69 cells. MCP‐1 antigen levels increased approximately 100‐fold in doxorubicin‐treated H69 cells. RT‐PCR using specific primer

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Author

Misako Shibakura; Kenji Niiya; Toru Kiguchi; Isao Kitajima; Masami Niiya; Noboru Asaumi; Nam-ho Huh; Yasunari Nakata; Mine Harada; Mitsune Tanimoto

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2002

Language

EN

Field

Medicine (Health Sciences)