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Normal B cells fail to secrete interleukin-12 by Jean-Charles Guéry; Francesco Ria; Francesca Galbiati; Luciano Adorini is a Immunology and Microbiology article available to read on EtoBox.

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Interleukin-12 is a key regulatory cytokine produced by antigen-presenting cells (APC) which drives the development of interferon-y (IFN-y)-producing cells and promotes cell-mediated immunity. Following subcutaneous immunization with protein antigen in adjuvant, dendritic cells (DC) but not small nor large B cells in immune lymph nodes express antigenic complexes and secrete substantial amounts of bioactive IL-12 p75 upon antigen-specific interaction with Tcells. We have analyzed secretion of IL-12 p40 and p75 by cell populations enriched in DC, macrophages or B cells in response to nonspecific stimulation or to interaction with antigen-specific CD4' cells. These APC populations do not produce IL-12 constitutively but, upon stimulation with heat-fixed Staphylococcus aureus and IFN-y, IL-12 p40 and p75 are secreted by DC and macrophages, whereas B cells fail to produce IL-12. B cells also fail to secrete IL-12 in response to stimulation with LPS and IFN-y. Co-culture with CD4' T hybridoma cells and antigen induces IL-12 secretion by DC. Up-regulation of IL-12 secretion by interaction with antigen-specific CD4' T cells is abrogated by anti-class I1 monoclonal antibodies (mAb), by sol

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Author

Jean-Charles Guéry; Francesco Ria; Francesca Galbiati; Luciano Adorini

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 0014-2980)

Published

1997

Language

EN

Field

Immunology and Microbiology (Life Sciences)