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Understanding Real-Time PCR Techniques by Harshad Malgani is a document available to read on EtoBox.

What is Understanding Real-Time PCR Techniques about?

This document discusses real-time PCR. Real-time PCR is a type of PCR that allows for detection and quantification of DNA during the amplification process rather than after completion. It involves adding fluorescent dyes or probes to the PCR reaction that bind to double-stranded DNA as it accumulates, allowing quantification of DNA at each cycle in the exponential phase. This provides advantages over traditional PCR like increased sensitivity and ability to detect small changes in DNA levels without needing

Author
Harshad Malgani
Language
EN

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