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Liquid Chromatographic–mass Spectrometric Determination of Cyclooxygenase Metabolites of Arachidonic Acid in Cultured Cells by Kasem Nithipatikom; Nathan D Laabs; Marilyn A Isbell; William B Campbell is a Chemistry article available to read on EtoBox.

What is Liquid Chromatographic–mass Spectrometric Determination of Cyclooxygenase Metabolites of Arachidonic Acid in Cultured Cells about?

A liquid chromatographic-electrospray ionization-mass spectrometric (LC-ESI-MS) technique was developed to simultaneously determine the cyclooxygenase metabolites of arachidonic acid (6-keto-PGF(1alpha), PGD(2), PGE(2), PGF(2alpha), and PGJ(2)) produced by cultured cells. Samples were separated on a C(18) column with water-acetonitrile mobile phase, ionized by electrospray, and detected in the positive mode. Selected ion monitoring (SIM) of m/z 353, 335, 335, 319, and 317 were used for quantifying 6-keto-PGF(1alpha), PGD(2), PGE(2), PGF(2alpha), and PGJ(2), respectively. Prostaglandins were detected at concentrations as low as 1 pg (S/N=3) on the column. The method was used to determine the production of PGs from bovine coronary artery endothelial cells (ECs) and human prostate cancer cells (PC-3) with different degree of invasiveness. Bradykinin (10(-6) M) stimulated a marked increase in the production of 6-keto-PGF(1alpha), PGE(2), and PGF(2alpha) and a small increase of PGD(2) by ECs. 6-Keto-PGF(1alpha) was the major metabolite in these cells. The production of PGE(2) was threefold higher and PGD(2) was twofold higher in PC-3-S (invasive) cells than in PC-3-U (non-invasive) cell

Who reads Liquid Chromatographic–mass Spectrometric Determination of Cyclooxygenase Metabolites of Arachidonic Acid in Cultured Cells?

Author

Kasem Nithipatikom; Nathan D Laabs; Marilyn A Isbell; William B Campbell

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-376X)

Published

2003

Language

EN

Field

Chemistry (Physical Sciences)