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Enzymatic Activity of Sperm Proprotein Convertase Is Important for Mammalian Fertilization by Sitthichai Iamsaard; Rapeepun Vanichviriyakit; Greanggrai Hommalai; Arpornrad Saewu; Nopparat Srakaew; Boonsirm Withyachumnarnkul; Ajoy Basak; Nongnuj Tanphaichitr is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Proprotein convertase subtilisin/kexin 4 (PCSK4) is implicated for sperm fertilizing ability, based on studies using __Pcsk4‐__null mice. Herein we demonstrated proprotein convertase (PC) activity in intact sperm and acrosomal vesicles. To determine whether this activity was important for sperm fertilizing ability, a peptide inhibitor was designed based on PCSK4 prodomain sequence (proPC4^75–90^), which contains its primary autocatalytic cleavage site. ProPC4^75–90^ inhibited recombinant PCSK4's activity with a __K__~i~ value of 5.4 μM, and at 500 μM, it inhibited sperm PC activity almost completely. Treatment of sperm with proPC4^75–90^ inhibited their egg fertilizing ability in a dose dependent manner. Correlation between sperm PC activity and fertilizing ability showed a high co‐efficient value (>0.9), indicating the importance of sperm PC activity in fertilization. In particular, sperm PC activity was important for capacitation and zona pellucida (ZP)‐induced acrosome reaction, since proPC4^75–90^‐treated sperm showed markedly decreased rates in these two events. These results were opposite to those observed in __Pcsk4‐__null sperm, which contained higher PC activit
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It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Sitthichai Iamsaard; Rapeepun Vanichviriyakit; Greanggrai Hommalai; Arpornrad Saewu; Nopparat Srakaew; Boonsirm Withyachumnarnkul; Ajoy Basak; Nongnuj Tanphaichitr
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)