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Prognostic significance of CD38 and CD20 expression as assessed by quantitative flow cytometry in chronic lymphocytic leukaemia by Eric D. Hsi; Kenneth J. Kopecky; Frederick R. Appelbaum; David Boldt; Tom Frey; Margaret Loftus; Mohamad A. Hussein is a Medicine article available to read on EtoBox.

What is Prognostic significance of CD38 and CD20 expression as assessed by quantitative flow cytometry in chronic lymphocytic leukaemia about?

**Summary.** CD38 expression on chronic lymphocytic leukaemia (CLL) cells is a poor prognostic factor, however, methods for measuring this vary. The QuantiBRITE^TM^ flow cytometry (FC) system yields an absolute antigen expression value (antibodies bound/cell, ABC) and may be useful in standardizing CD38 expression analysis. We evaluated cryopreserved pretreatment CLL cells for CD20 ABC, CD38 ABC, and percentage of CD38^+^ B cells from 131 patients requiring therapy. The 92 patients (70%) with ≥ 100 CD38 ABC had worse overall survival (OS; 34% at 5 years) compared with those with < 100 CD38 ABC (70% at 5 years, mortality hazard ratio 2·30, 95% confidence interval 1·28–4·12; two‐tailed __P__ = 0·003). Among the 64 patients with < 30% CD38^+^ cells, OS of the 25 with ≥ 100 ABC was worse than that of the 39 with < 100 ABC (__P =__ 0·018). OS of patients with < 30% CD38^+^ cells and ≥ 100 ABC was actually similar to that of patients with ≥ 30% CD38^+^ cells. ^Bright^CD20 expression (≥ 20 000 ABC) was not associated with a worse OS (__P =__ 0·10). The presence of ≥ 100 CD38 ABC in CLL patients requiring therapy is an unfavourable prognostic factor for OS and quantitative FC may be superi

Who reads Prognostic significance of CD38 and CD20 expression as assessed by quantitative flow cytometry in chronic lymphocytic leukaemia?

Author

Eric D. Hsi; Kenneth J. Kopecky; Frederick R. Appelbaum; David Boldt; Tom Frey; Margaret Loftus; Mohamad A. Hussein

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

2003

Language

EN

Field

Medicine (Health Sciences)