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Can I read RT-PCR detection of SARS-CoV-2 in nasopharyngeal and salivary specimens: contribution of alternative collection systems and extraction processes to cope with mass screening. Interpretation of low viral loads on EtoBox?
RT-PCR detection of SARS-CoV-2 in nasopharyngeal and salivary specimens: contribution of alternative collection systems and extraction processes to cope with mass screening. Interpretation of low viral loads by Sylvain Robinet; François Parisot; Laurie Cochonot; Benjamin Schiltz; Camille Paboeuf; Clement Nedelec; Laurent Espinet; Alexis Heddebaut is a Medicine article available to read on EtoBox.
What is RT-PCR detection of SARS-CoV-2 in nasopharyngeal and salivary specimens: contribution of alternative collection systems and extraction processes to cope with mass screening. Interpretation of low viral loads about?
## Abstract ## Objectives Due to massive screening of the persistent coronavirus SARS-CoV-2, supply difficulties emerged for swabs and extraction reagents leading to test alternative choices. Quality sampling may have an impact on the result and a low RNA detection may be difficult to interpret because it does not necessarily mean that infectious particles are present in biological samples. There is a need to understand whether the Ct value information is relevant and informative. ## Methods We compared the pre-analytical stability of RNA in saline solution, UTM^®^, Amies and Cary-Blair transport media. Expression profile of E, N and RdRp genes was assessed at various concentration levels with the AllplexTM 2019-nCoV Assay. Factors that may influence the determination of Ct were studied with several extraction reagents coupled to the GSD NovaPrime^®^ SARS-CoV-2 RT-PCR testing kit. ## Results Seventy two-hour RNA stability has been demonstrated for all the transport media assessed. A matrix effect was shown, leading to a decrease in the detection of E and RdRp genes, so that only N gene was often found for Ct greater than 35.0. A follow-up over more than 67,000 patients suggests tha
Who reads RT-PCR detection of SARS-CoV-2 in nasopharyngeal and salivary specimens: contribution of alternative collection systems and extraction processes to cope with mass screening. Interpretation of low viral loads?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Sylvain Robinet; François Parisot; Laurie Cochonot; Benjamin Schiltz; Camille Paboeuf; Clement Nedelec; Laurent Espinet; Alexis Heddebaut
- Publisher
- Walter de Gruyter GmbH
- Published
- 2022
- Language
- EN
- Field
- Medicine (Health Sciences)