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Two-photon Fluorescence Lifetime Imaging Microscopy of Macrophage-mediated Antigen Processing by T. French; P. T. C. So; D. J. Weaver; Jr; T. Coelho-Sampaio; E. Gratton; E. W. Voss; Jr; J. Carrero is a Medicine article available to read on EtoBox.
What is Two-photon Fluorescence Lifetime Imaging Microscopy of Macrophage-mediated Antigen Processing about?
Two‐photon fluorescence lifetime imaging microscopy was used noninvasively to monitor a fluorescent antigen during macrophage‐mediated endocytosis, intracellular vacuolar encapsulation, and protease‐dependent processing. Fluorescein‐conjugated bovine serum albumin (FITC–BSA) served as the soluble exogenous antigen. As a relatively nonfluorescent probe in the native state, the antigen was designed to reflect sequential intracellular antigen processing events through time‐dependent changes in fluorescence properties. Using two‐photon lifetime imaging microscopy, antigen processing events were monitored continuously for several hours. During this time, the initial fluorescein fluorescence lifetime of 0.5 ns increased to α 3.0 ns. Control experiments using fluorescein conjugated poly‐l‐lysine and poly‐d‐lysine demonstrated that the increase in fluorescence parameters observed with FITC–BSA were due to intracellular proteolysis since addition of the inert d‐isomer did not promote an increase in fluorescence lifetime or intensity. Comparisons of intravacuolar and extracellular FITC–dextran concentration suggested active localization of dextran in the vacuoles by the macrophage. In additi
Who reads Two-photon Fluorescence Lifetime Imaging Microscopy of Macrophage-mediated Antigen Processing?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- T. French; P. T. C. So; D. J. Weaver; Jr; T. Coelho-Sampaio; E. Gratton; E. W. Voss; Jr; J. Carrero
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley-Blackwell Publishing Ltd; Wiley (ISSN 0022-2720)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Physical Sciences)