Can I read An Oxidative Stress-specific Bacterial Cell Array Chip for Toxicity Analysis on EtoBox?

An Oxidative Stress-specific Bacterial Cell Array Chip for Toxicity Analysis by Jin Hyung Lee; Chul Hee Youn; Byoung Chan Kim; Man Bock Gu is a Engineering article available to read on EtoBox.

What is An Oxidative Stress-specific Bacterial Cell Array Chip for Toxicity Analysis about?

An oxidative stress-specific bacterial cell array chip was fabricated and implemented in the analysis of various different chemicals. The chip consisted of twelve toxicity responsive strains that respond specifically to different oxidative toxicities such as the generation of the superoxide radical, except for strain EBMalK, which was included as a negative control. Each bioluminescent strain carried a fusion of a stress gene promoter (sodA, pqi-5, soxR, fumC, soxS, inaA, hmp, malK, katG, zwf, fpr or pgi) to the bacterial lux reporter genes. A total of nine chemicals were selected to exhibit the capabilities of this array when analyzing different oxidative toxicities. Each of the chemicals were categorized according to their structure and their ability to form radicals in vivo: (I) paraquat, an active radical producer, (II) structural analogs of paraquat that produce radicals, (III) chemicals that are distinct from paraquat but still produce radicals and (IV) chemicals having similar structures as paraquat but do not produce radicals. The results found that each strain was responsive to one or more of the compounds tested but, as a definitive factor, the responses from the chip wer

Who reads An Oxidative Stress-specific Bacterial Cell Array Chip for Toxicity Analysis?

Author

Jin Hyung Lee; Chul Hee Youn; Byoung Chan Kim; Man Bock Gu

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)