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Determination of the mycotoxin moniliformin in cultures of Fusarium subglutinans and in naturally contaminated maize by high-performance liquid chromatography–atmospheric pressure chemical ionization mass spectrometry by V Sewram; T.W Nieuwoudt; W.F.O Marasas; G.S Shephard; A Ritieni is a Chemistry article available to read on EtoBox.

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A LC-MS method employing triethylamine as ion-pairing reagent for the determination of moniliformin in culture material and naturally contaminated maize samples is described. Mass spectrometric detection of moniliformin was 2 accomplished following atmospheric pressure chemical ionization to yield the deprotonated molecular ion [M2H] at m /z 97. The moniliformin response was found to be linear over the injected range 10 ng to 700 ng and a detection limit of 10 ng was attainable at a signal-to-noise (S /N) ratio of 4. Five South African strains of Fusarium subglutinans were grown on maize kernels and moniliformin extracted with an acetonitrile-water (95:5) mixture. Following sample clean up with reversed-phase (C ) solid-phase extraction cartridges, the extracts were subjected to LC-MS analysis. Triethylamine was 18 used as an ion-pair reagent and found to improve the retention characteristics of moniliformin without any detrimental effects to the instrument. Moniliformin concentrations ranged between 130 mg / kg and 1460 mg / kg culture. Application of this method to naturally contaminated maize samples from Transkei showed that it was capable of measuring moniliformin levels down

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Author

V Sewram; T.W Nieuwoudt; W.F.O Marasas; G.S Shephard; A Ritieni

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1999

Language

EN

Field

Chemistry (Physical Sciences)