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Involvement of CSF‐1 in generating a stroma‐independent hematopoietic stem cell line by Christoph Heberlein; Jutta Friel; Katsuhiko Itoh; Eugene Medlock; Luke Li; Naoki Nakayama; Carol Stocking; Maren Geldmacher; Wolfram Ostertag is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The hematopoietic stem cell line, Myl‐D7, is maintained by a self‐renewing stem cell population that spontaneously generates myeloid, lymphoid, and erythroid progeny. MS‐5 stromal cells are necessary for the growth of Myl‐D7 cells. One component of the Myl‐D7 cells proliferation activity released by MS‐5 stromal cells was enriched by Q sepharose fractionation and shown to be colony stimulating factor‐1 (CSF‐1) by Western blotting, BAC1.2F5 cell bioassay and inhibition of Myl‐D7 proliferation by CSF‐1 antibody. The requirement of Myl‐D7 cells for CSF‐1 was also demonstrated independently by selecting for rare, stroma‐independent Myl‐D7 mutant clones able to grow without stroma and additional factors. Eighty‐nine stroma‐independent mutant clones were obtained and belonged to two classes. The majority of mutants did not secrete any growth promoting activity. The second, rarer class of mutants releases a factor that stimulates proliferation/survival for up to several months and approximately half of the secretors express high levels of CSF‐1 mRNA. Wild type Myl‐D7 grown with supernatants from the secretor cells retained the stem cell phenotype. These data suggest that CSF‐1

Who reads Involvement of CSF‐1 in generating a stroma‐independent hematopoietic stem cell line?

Author

Christoph Heberlein; Jutta Friel; Katsuhiko Itoh; Eugene Medlock; Luke Li; Naoki Nakayama; Carol Stocking; Maren Geldmacher; Wolfram Ostertag

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)