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Formation and structural determinants of multi-stranded guanine-rich DNA complexes by Karen Poon; Robert B. Macgregor Jr. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Formation and structural determinants of multi-stranded guanine-rich DNA complexes about?

We have investigated the complexes formed by oligonucleotides with the general sequence d(T15,Gn), where n = 4-15. Two distinct classes of structures are formed, namely, the four-stranded tetraplex and frayed wires. Frayed wires differ from four-stranded tetraplexes in both strand association stoichiometry and the ability of dimethyl sulfate to methylate the N7 position of guanine. Thus, it appears that these two guanine-rich multistranded assemblies are stabilised by different guanine-guanine interactions. The number of contiguous guanine residues determines which of the complexes is favoured. Based on the stoichiometry of the associated species and the accessibility of the N7 position of guanine to methylation we have found that oligonucleotides with smaller number of contiguous guanines; n = 5-8, form primarily four-stranded tetraplex. Oligonucleotides with larger numbers of contiguous guanines adapt primarily the frayed wire structure. The stability of the complexes formed by this series of oligonucleotides is determined by the number and arrangement of the guanines within the sequences. We propose that the formation of the two types of complex proceed by a parallel reaction pa

Who reads Formation and structural determinants of multi-stranded guanine-rich DNA complexes?

Author

Karen Poon; Robert B. Macgregor Jr.

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)