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Release of Prostacyclin, Endothelium-derived Relaxing Factor and Endothelin by Freshly Harvested Cells Attached to Microcarrier Beads by S. Kibira; R. Dudek; K. S. Narayan; R. J. Bing is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Release of Prostacyclin, Endothelium-derived Relaxing Factor and Endothelin by Freshly Harvested Cells Attached to Microcarrier Beads about?

Cultured endothelial cells have been used in the past as a source of endothelium-derived relaxing factor (EDRF) and of prostacyclin (PGI2). Although cell cultures are essential for observation of prolonged exposure to media or when there is delayed response, they are time consuming and sterile conditions are essential. In the present study, we report that endothelial cells, freshly harvested from bovine aortas, readily attached themselves to cytodex-3 microcarrier beads and released an endothelium-derived relaxing factor (EDRF), prostacyclin (PGI2) and increased the amount of cyclic GMP in vascular smooth muscle. Attachment to microcarrier beads was essential since it increased the surface area and the number of attached cells and permitted collection of cell free filtrates because of the formation of dense networks of cells and beads. As a result superfusion of cells and beads on the filter did not dislodge bound cells which remain on the filter. Conditioned filtrates from freshly harvested endothelial cells attached to microcarrier beads caused marked relaxation of endothelium-deprived bovine pulmonary artery strips. The degree of relaxation depended on the number of cells; maxim

Who reads Release of Prostacyclin, Endothelium-derived Relaxing Factor and Endothelin by Freshly Harvested Cells Attached to Microcarrier Beads?

Author

S. Kibira; R. Dudek; K. S. Narayan; R. J. Bing

Publisher

Springer; Springer-Verlag; Kluwer Academic/Plenum Publishers; Springer Science and Business Media LLC (ISSN 0300-8177)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)