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Effect of microinjectecd calcium-calmodulin on mitosis in PtK2 cells by Charles H. Keith is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Calcium and calmodulin are believed to play a significant role in the regulation of mitosis, because they are both localized in the mitotic spindle and because they can potentiate microtubule depolymerization in the test tube and in the living cell. It has been hypothesized, specifically, that calcium-saturated calmodulin drives the shortening of the kinetochore microtubules that must occur during prometaphase, when the chromosomes congress to the metaphase plate, and during anaphase A, when the half-spindles shorten. We have examined the role of calmodulin in mitosis by observing the consequences of calmodulin microinjection on the progress of mitosis and morphology of the mitotic spindle in PtK2 cells. We have found that the injection of excess calcium-saturated calmodulin during early prometaphase significantly prolongs the time required for the cell to go into anaphase, and that neither calcium-depleted calmodulin nor buffer alone produce a similar perturbation. Calcium ion alone produces a similar but much smaller retardation of mitosis. Immunofluorescence and fluorescent analogue cytochemical studies of spindle morphology reveal that the immediate (< 5-min) effect of calcium-

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Author

Charles H. Keith

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0886-1544)

Published

1987

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)