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## Abstract Reversed phase liquid chromatography was combined with the multiple stage mass analysis capability of an ion trap mass spectrometer for the characterization of permethylated oligosaccharide mixtures. The new method was used to separate the components of an unlabeled permethylated maltooligomer ladder, a 2‐aminobenzamide‐labeled (2‐AB) maltooligomer ladder, a complex mixture of 2AB‐labeled bi‐ (B), tri‐ (T), and tetraantennary (Q) standards, and a mixture of recombinant glycoprotein carbohydrates from __soluble__ CD4 with varying sialic acid (S) content. Using reversed phase HPLC, permethylated mixture components including α and β anomers were separated based on their structures. Fluorescent labeling with 2‐aminobenzamide prior to permethylation was employed for off‐line method development, but was not necessarily required for mass spectral analysis, as permethylation alone improved the ionization and fragmentation characteristics of the molecules. Antennae composition of permethylated derivatives was determined in MS^2^ where the fragmentation patterns of the Y‐ and B‐ion series predominated, and then further evaluated in MS^3^, which provided additional information on

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Author

Jeannine Delaney; Paul Vouros

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2001

Language

EN

Field

Chemistry (Physical Sciences)