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Interleukin‐1β Selectively Decreases the Synthesis of Versican by Arterial Smooth Muscle Cells by Joan M. Lemire; Christina K. Chan; Steven Bressler; John Miller; Richard G. LeBaron; Thomas N. Wight is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Proteoglycans accumulate in lesions of atherosclerosis but little is known as to which factors regulate the synthesis of these molecules. Interleukin‐1β (IL‐1β) is a cytokine involved in vascular lesion development but it is not clear whether it has specific effects on proteoglycan synthesis by arterial smooth muscle cells (ASMC). Monkey ASMC were treated with IL‐1β and proteoglycan synthesis assessed using [^35^S]‐sulfate and [^35^S]‐Trans amino acid labeling. Four prominent size populations of proteoglycans, as determined by SDS–PAGE gradient gel electrophoresis, were observed in the culture medium and identified as versican, biglycan, decorin, and an unknown population that migrated to the gel interface. IL‐1β treatment decreased significantly the synthesis of versican, while increasing the synthesis of decorin, but having no effect on biglycan synthesis. Northern blot analyses confirmed this selective effect on versican and decorin mRNA transcripts. Nuclear run‐on and RNA inhibition studies showed that decreased mRNA for versican was due to increased mRNA degradation and not to changes in transcription. In addition, IL‐1β increased the synthesis of the population of
Who reads Interleukin‐1β Selectively Decreases the Synthesis of Versican by Arterial Smooth Muscle Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Joan M. Lemire; Christina K. Chan; Steven Bressler; John Miller; Richard G. LeBaron; Thomas N. Wight
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)