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Rapeseed and Milk Protein Exhibit a Similar Overall Nutritional Value but Marked Difference in Postprandial Regional Nitrogen Utilization in Rats by Claire Boutry; Hélène Fouillet; François Mariotti; François Blachier; Daniel Tomé; Cécile Bos is a Medicine article available to read on EtoBox.
What is Rapeseed and Milk Protein Exhibit a Similar Overall Nutritional Value but Marked Difference in Postprandial Regional Nitrogen Utilization in Rats about?
## Background Rapeseed is an emerging and promising source of dietary protein for human nutrition and health. We previously found that rapeseed protein displayed atypical nutritional properties in humans, characterized by low bioavailability and a high postprandial biological value. The objective of the present study was to investigate the metabolic fate of rapeseed protein isolate (RPI) and its effect on protein fractional synthesis rates (FSR) in various tissues when compared to a milk protein isolate (MPI). ## Methods Rats (n = 48) were given a RPI or MPI meal, either for the first time or after 2-week adaptation to a MPI or RPI-based diet. They were divided in two groups for measuring the fed-state tissue FSR 2 h after the meal (using a flooding dose of ^13^C-valine) and the dietary N postprandial distribution at 5 h (using ^15^N-labeled meals). ## Results RPI and MPI led to similar FSR and dietary nitrogen (N) losses (ileal and deamination losses of 4% and 12% of the meal, respectively). By contrast, the dietary N incorporation was significantly higher in the intestinal mucosa and liver (+36% and +16%, respectively) and lower in skin (-24%) after RPI than MPI. ## Conclusions A
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- Author
- Claire Boutry; Hélène Fouillet; François Mariotti; François Blachier; Daniel Tomé; Cécile Bos
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2004-; Springer Science and Business Media LLC (ISSN 1743-7075)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)