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This document describes methods for estimating different types of tannins in samples. It involves extracting tannins from dried and ground samples using diethyl ether and acetone. Total phenols are then estimated using a Folin Ciocalteu reagent and reading absorbance. Non-tannin phenols are also measured after precipitating tannins using polyvinyl polypyrrolidone. Condensed tannins are measured using a butanol-HCl and ferric reagent, while hydrolysable tannins are calculated by subtracting condensed tannins

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