Can I read Contractility of Single Human Dermal Myofibroblasts and Fibroblasts on EtoBox?

Contractility of Single Human Dermal Myofibroblasts and Fibroblasts by Louise K. Wrobel; Tim R. Fray; Justin E. Molloy; Julian J. Adams; Mark P. Armitage; John C. Sparrow is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Contractility of Single Human Dermal Myofibroblasts and Fibroblasts about?

Human dermal myofibroblasts, characterised by the expression of alpha-smooth muscle actin, are part of the granulation tissue and implicated in the generation of contractile forces during normal wound healing and pathological contractures. We have compared the contractile properties of single human dermal fibroblasts and human dermal myofibroblasts by culturing them on flexible silicone elastomers. The flexibility of the silicone substratum permits the contractile forces exerted by the cells to be measured [Fray et al., 1998: Tissue Eng. 4:273-283], without changing their expression of alpha-smooth muscle actin. The mean contractile force produced by myofibroblasts (2.2 microN per cell) was not significantly different from that generated by fibroblasts (2.0 microN per cell) when cultured on a substrata with a low elastomer stiffness. Forces produced by fibroblasts were unaffected by increases in elastomer stiffness, but forces measured for myofibroblasts increased to a mean value of 4.1 microN/cell. This was associated with a higher proportion of myofibroblasts being able to produce wrinkles on elastomers of high stiffness compared to fibroblasts. We discuss the force measurements

Who reads Contractility of Single Human Dermal Myofibroblasts and Fibroblasts?

Author

Louise K. Wrobel; Tim R. Fray; Justin E. Molloy; Julian J. Adams; Mark P. Armitage; John C. Sparrow

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0886-1544)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)