Can I read Distribution of Methyl Substituents in Amylose and Amylopectin from Methylated Potato Starches on EtoBox?

Distribution of Methyl Substituents in Amylose and Amylopectin from Methylated Potato Starches by Yuri E.M. van der Burgt; Jack Bergsma; Ido P. Bleeker; Paul J.H.C. Mijland; Anca van der Kerk-van Hoof; Johannis P. Kamerling; Johannes F.G. Vliegenthart is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Distribution of Methyl Substituents in Amylose and Amylopectin from Methylated Potato Starches about?

Granular potato starches were methylated in aqueous suspension with dimethyl sulfate to molar substitution (MS) values up to 0.29. Fractions containing mainly amylose or amylopectin were obtained after aqueous leaching of the derivatised starch granules. Amylopectin in these fractions was precipitated with Concanavalin A to separate it from amylose. Amylose remained in solution and was enzymatically converted into D-glucose for quantification, thereby taking into account the decreased digestibility due to the presence of methyl substituents. It was found that the MS of amylose was 1.6-1.9 times higher than that of amylopectin in methylated starch granules. The distributions of methyl substituents in trimers and tetramers, prepared from amylose- or amylopectin-enriched fractions, were determined by FAB mass spectrometry and compared with the outcome of a statistically random distribution. It turned out that substituents in amylopectin were distributed heterogeneously, whereas substitution of amylose was almost random. The results are rationalised on the basis of an organised framework that is built up from amylopectin side chains. The crystalline lamellae are less accessible for sub

Who reads Distribution of Methyl Substituents in Amylose and Amylopectin from Methylated Potato Starches?

Author

Yuri E.M. van der Burgt; Jack Bergsma; Ido P. Bleeker; Paul J.H.C. Mijland; Anca van der Kerk-van Hoof; Johannis P. Kamerling; Johannes F.G. Vliegenthart

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0008-6215)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)