Opening book details…
Can I read Tubulin Engineering by Semi-synthesis Reveals That Polyglutamylation Directs Detyrosination on EtoBox?
Tubulin Engineering by Semi-synthesis Reveals That Polyglutamylation Directs Detyrosination by Eduard Ebberink; Simon Fernandes; Georgios Hatzopoulos; Ninad Agashe; Po-Han Chang; Nora Guidotti; Timothy M. Reichart; Luc Reymond; Marie-Claire Velluz; Fabian Schneider; Cédric Pourroy; Carsten Janke; Pierre Gönczy; Beat Fierz; Charlotte Aumeier is a Chemistry article available to read on EtoBox.
What is Tubulin Engineering by Semi-synthesis Reveals That Polyglutamylation Directs Detyrosination about?
Microtubules, a critical component of the cytoskeleton, carry post-translational modifications (PTMs) that are important for the regulation of key cellular processes. Long-lived microtubules, in neurons particularly, exhibit both detyrosination of α-tubulin and polyglutamylation. Dysregulation of these PTMs can result in developmental defects and neurodegeneration. Owing to a lack of tools to study the regulation and function of these PTMs, the mechanisms that govern such PTM patterns are not well understood. Here we produce fully functional tubulin carrying precisely defined PTMs within its C-terminal tail. We ligate synthetic α-tubulin tails-which are site-specifically glutamylatedto recombinant human tubulin heterodimers by applying a sortase-and intein-mediated tandem transamidation strategy. Using microtubules reconstituted with these designer tubulins, we find that α-tubulin polyglutamylation promotes its detyrosination by enhancing the activity of the tubulin t yr os ine c ar bo xy pe pt idase v as oh ib in /small vasohibin-binding protein in a manner dependent on the length of polyglutamyl chains. We also find that modulating polyglutamylation levels in cells results in cor
Who reads Tubulin Engineering by Semi-synthesis Reveals That Polyglutamylation Directs Detyrosination?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Eduard Ebberink; Simon Fernandes; Georgios Hatzopoulos; Ninad Agashe; Po-Han Chang; Nora Guidotti; Timothy M. Reichart; Luc Reymond; Marie-Claire Velluz; Fabian Schneider; Cédric Pourroy; Carsten Janke; Pierre Gönczy; Beat Fierz; Charlotte Aumeier
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Chemistry (Physical Sciences)