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Metabolism of low‐density lipoprotein in differentiated and undifferentiated HT29 colon cancer cells by Viviane Viallard; Christiane Lacombe; Véronique Trocheris; Christiane Tabacik; Sigrid Aliau is a Medicine article available to read on EtoBox.
What is Metabolism of low‐density lipoprotein in differentiated and undifferentiated HT29 colon cancer cells about?
## Abstract The metabolism of human low‐density lipoproteins was studied in 2 subpopulations deriving from cells of HT29, a human colon carcinoma cell line. When grown on standard medium (25 mM glucose), about 95% of these cells are undifferentiated (G^+^ cells). From this heterogeneous population, a subpopulation with features of differentiated small‐intestinal cells was selected by glucose deprivation (G^−^ cells). The characteristics of the LDL receptor were first investigated. The results showed that the binding of ^125^I‐LDL to G^+^ and G^−^ cells performed at 4°C was saturable and specific. The K~d~values were not statistically different in the 2 cell subpopulations. The B~max~ of G^+^ cells was 55 ± 6 ng ^125^I‐LDL/mg cell protein and showed no changes whatever the phase of culture. In G^−^ cells, the B~max~ was higher during the exponential phase of culture and decreased in the post‐confluent phase (82 ± 5 versus 15 ± 6.8 ng ^125^I‐LDL/mg cell protein). Cellular degradation of ^125^I‐LDL was effective in both cell subpopulations but time‐course studies showed that, in post‐confluent G^−^ cells, degradation was slowed as compared to G^+^ cells (4 hr vs. 2 hr to reach maximal
Who reads Metabolism of low‐density lipoprotein in differentiated and undifferentiated HT29 colon cancer cells?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Viviane Viallard; Christiane Lacombe; Véronique Trocheris; Christiane Tabacik; Sigrid Aliau
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1990
- Language
- EN
- Field
- Medicine (Health Sciences)