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A simple quantitative assay of 125I-labeled α-bungarotoxin binding to soluble and membrane-bound acetylcholine receptor protein by Ronald A. Kohanski; John P. Andrews; Pierre Wins; Mohyee E. Eldefrawi; George P. Hess is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A simple quantitative assay of 125I-labeled α-bungarotoxin binding to soluble and membrane-bound acetylcholine receptor protein about?
This method involves the irreversible formation of a complex between ?labeled cu-bungarotoxin and the acetylcholine receptor in either its membranebound or purified state. The separation of the labeled toxin-receptor complex from unreacted toxin is accomplished by chromatography on carboxymethylcellulose cation-exchange resin. The method described was developed to satisfy the following experimental requirements that could not be dealt with in their entirety by employing any of the published methods: (i) the complete recovery of reacted and unreacted species in relatively small volumes; (ii) an efficient and precise isolation of the specific and irreversible '\*Wabeled cr-bungarotoxin-receptor complex when the complexation reactions demand a large excess of unlabeled a-bungarotoxin for quenching (a 20-fold molar excess of unlabeled over labeled toxin); (iii) this isolation of the toxin-receptor complex allows one to determine the protein concentrations in the samples, a necessity in experiments covering a wide range of receptor concentrations: (iv) a consistent low blank for binding site concentrations ranging over two or three orders of magnitude: (v) simplicity and rapidity.
Who reads A simple quantitative assay of 125I-labeled α-bungarotoxin binding to soluble and membrane-bound acetylcholine receptor protein?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ronald A. Kohanski; John P. Andrews; Pierre Wins; Mohyee E. Eldefrawi; George P. Hess
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1977
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)