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Can I read Galactosylated Multimodular Lipoplexes for Specific Gene Transfer Into Primary Hepatocytes on EtoBox?
Galactosylated Multimodular Lipoplexes for Specific Gene Transfer Into Primary Hepatocytes by Emilie Letrou‐Bonneval; Raphaël Chèvre; Olivier Lambert; Philippe Costet; Corinne André; Charles Tellier; Bruno Pitard is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Galactosylated Multimodular Lipoplexes for Specific Gene Transfer Into Primary Hepatocytes about?
## Abstract ## Background Numerous synthetic cationic vectors have been synthesized and are successfully used for __in vitro__ gene transfer but an excess of positive charges can lead to cytotoxicity and does not enable specific transfection. ## Methods We decided to develop alternative molecular systems consisting of neutral, colloidally stable bioassemblies equipped with ligands for specific cell targeting. Consequently, we directed our efforts toward the development of a multimodular non‐viral gene delivery system consisting of a condensed core of DNA with cationic liposomes of bis(guanidinium)‐tren‐cholesterol and an external corona of poly(ethylene oxide) stretches harbored by the steric stabilizers used to stabilize lipoplexes colloidally. A ligand capable of cell targeting by receptor‐mediated endocytosis was covalently linked at the poly(ethylene oxide) extremity of steric stabilizers. Steric stabilizers were functionalized by a one‐step enzymatic galactosylation to develop new supramolecular assemblies of lipoplexes able to target asialoglycoprotein receptors located on primary hepatocytes. ## Results Cryo‐TEM and fluorescence experiments showed that DNA was condensed with
Who reads Galactosylated Multimodular Lipoplexes for Specific Gene Transfer Into Primary Hepatocytes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Emilie Letrou‐Bonneval; Raphaël Chèvre; Olivier Lambert; Philippe Costet; Corinne André; Charles Tellier; Bruno Pitard
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)